38.5 Folate (categories 2)

Folate range (<40 vs >=40)

38.5.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	27.6439848	1	<0.0001	***
Folate_gp2	0.6834872	1	0.4	ns
Study	0.6172392	1	0.4	ns
GA_samp	20.6035106	1	<0.0001	***
Folate_gp2:Study	0.0906157	1	0.8	ns

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	5.1369360	1	0.02	*
Folate_gp2	0.4004384	1	0.5	ns
Study	0.5113037	1	0.5	ns
GA_samp	312.9945968	1	<0.0001	***
Folate_gp2:Study	1.1235867	1	0.3	ns

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	29.6153063	1	<0.0001	***
Folate_gp2	0.2043583	1	0.7	ns
Study	0.0349007	1	0.9	ns
GA_samp	95.2457244	1	<0.0001	***
Folate_gp2:Study	1.0402265	1	0.3	ns

Insulin (uU/ml)

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp2	1	Inf	2.526	2.526	0.1	ns
3	Study	1	Inf	4.145	4.145	0.04	*
4	GA_samp	1	Inf	0.850	0.850	0.4	ns
2	Folate_gp2:Study	1	Inf	0.158	0.158	0.7	ns

HOMA-IR

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp2	1	Inf	2.907	2.907	0.09	ns
3	Study	1	Inf	7.737	7.737	0.005	**
4	GA_samp	1	Inf	0.384	0.384	0.5	ns
2	Folate_gp2:Study	1	Inf	0.184	0.184	0.7	ns

Glucose (mmol/L)

	model term	df1	df2	F.ratio	Chisq	p.value
1	Folate_gp2	1	Inf	0.810	0.810	0.4	ns
3	Study	1	Inf	28.313	28.313	<0.0001	***
4	GA_samp	1	Inf	0.623	0.623	0.4	ns
2	Folate_gp2:Study	1	Inf	0.099	0.099	0.8	ns

38.5.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	14.15 (5.63, 35.42)	<0.0001
Folate_gp2≥40	1.13 (0.95, 1.34)	0.19
StudySTOP	1.14 (0.83, 1.57)	0.43
GA_samp	1.14 (1.08, 1.20)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.68 (1.00, 2.78)	0.04
Folate_gp2≥40	1.04 (0.94, 1.15)	0.47
StudySTOP	1.20 (1.00, 1.44)	0.05
GA_samp	1.33 (1.29, 1.38)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.17 (0.09, 0.33)	<0.0001
Folate_gp2≥40	0.92 (0.81, 1.06)	0.25
StudySTOP	0.92 (0.72, 1.18)	0.52
GA_samp	1.22 (1.17, 1.27)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	20.52 (5.45, 77.29)	<0.0001
Folate_gp2≥40	0.79 (0.62, 1.02)	0.08
StudySTOP	0.62 (0.39, 0.99)	0.05
GA_samp	1.04 (0.96, 1.13)	0.34

HOMA-IR

Var	Effect	P
(Intercept)	6.49 (1.56, 26.94)	0.01
Folate_gp2≥40	0.76 (0.57, 1.00)	0.05
StudySTOP	0.49 (0.30, 0.81)	0.005
GA_samp	1.03 (0.94, 1.13)	0.51

Glucose (mmol/L)

Var	Effect	P
(Intercept)	6.85 (5.37, 8.74)	<0.0001
Folate_gp2≥40	0.96 (0.88, 1.04)	0.30
StudySTOP	0.79 (0.73, 0.86)	<0.0001
GA_samp	0.99 (0.97, 1.01)	0.53

38.5.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	70.73103	14.73149	52.79746	46.57671	107.4116
STOP	80.59937	13.34623	22.89233	57.21742	113.5364

hPL (μg/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	61.21751	4.864402	119.13250	52.30512	71.64849
STOP	73.28061	3.084380	30.03457	67.24489	79.85809

GH2 (ng/ml)

Study	response	SE	df	lower.CL	upper.CL
SCOPE	1.900528	0.2060202	135.66754	1.533812	2.354921
STOP	1.754287	0.0924152	39.19691	1.577001	1.951503

Insulin (uU/ml)

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	29.75754	5.993665	Inf	20.05168	44.16144
STOP	18.50744	1.470135	Inf	15.83913	21.62526

HOMA-IR

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	8.117903	1.7585737	Inf	5.309442	12.411916
STOP	3.980335	0.3401272	Inf	3.366532	4.706048

Glucose (mmol/L)

Study	response	SE	df	asymp.LCL	asymp.UCL
SCOPE	6.143411	0.2573602	Inf	5.659147	6.669114
STOP	4.856323	0.1075299	Inf	4.650075	5.071717

38.5.4 Comparisons (Overall)

Hormone	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	≥40 / <40	1.13 (0.94, 1.34)	0.2
hPL (μg/ml)	≥40 / <40	1.04 (0.94, 1.16)	0.5
GH2 (ng/ml)	≥40 / <40	0.92 (0.80, 1.06)	0.3
Insulin (uU/ml)	≥40 / <40	0.79 (0.62, 1.02)	0.08
HOMA-IR	≥40 / <40	0.76 (0.57, 1.00)	0.05
Glucose (mmol/L)	≥40 / <40	0.96 (0.88, 1.04)	0.3

38.5.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.5.6 Plots