38.5 Folate (categories 2)

Folate range (<40 vs >=40)

38.5.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 27.6439848 1 <0.0001 ***
Folate_gp2 0.6834872 1 0.4 ns
Study 0.6172392 1 0.4 ns
GA_samp 20.6035106 1 <0.0001 ***
Folate_gp2:Study 0.0906157 1 0.8 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 5.1369360 1 0.02 *
Folate_gp2 0.4004384 1 0.5 ns
Study 0.5113037 1 0.5 ns
GA_samp 312.9945968 1 <0.0001 ***
Folate_gp2:Study 1.1235867 1 0.3 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 29.6153063 1 <0.0001 ***
Folate_gp2 0.2043583 1 0.7 ns
Study 0.0349007 1 0.9 ns
GA_samp 95.2457244 1 <0.0001 ***
Folate_gp2:Study 1.0402265 1 0.3 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 2.526 2.526 0.1 ns
3 Study 1 Inf 4.145 4.145 0.04 *
4 GA_samp 1 Inf 0.850 0.850 0.4 ns
2 Folate_gp2:Study 1 Inf 0.158 0.158 0.7 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 2.907 2.907 0.09 ns
3 Study 1 Inf 7.737 7.737 0.005 **
4 GA_samp 1 Inf 0.384 0.384 0.5 ns
2 Folate_gp2:Study 1 Inf 0.184 0.184 0.7 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate_gp2 1 Inf 0.810 0.810 0.4 ns
3 Study 1 Inf 28.313 28.313 <0.0001 ***
4 GA_samp 1 Inf 0.623 0.623 0.4 ns
2 Folate_gp2:Study 1 Inf 0.099 0.099 0.8 ns

38.5.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 14.15 (5.63, 35.42) <0.0001
Folate_gp2≥40 1.13 (0.95, 1.34) 0.19
StudySTOP 1.14 (0.83, 1.57) 0.43
GA_samp 1.14 (1.08, 1.20) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.68 (1.00, 2.78) 0.04
Folate_gp2≥40 1.04 (0.94, 1.15) 0.47
StudySTOP 1.20 (1.00, 1.44) 0.05
GA_samp 1.33 (1.29, 1.38) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.17 (0.09, 0.33) <0.0001
Folate_gp2≥40 0.92 (0.81, 1.06) 0.25
StudySTOP 0.92 (0.72, 1.18) 0.52
GA_samp 1.22 (1.17, 1.27) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 20.52 (5.45, 77.29) <0.0001
Folate_gp2≥40 0.79 (0.62, 1.02) 0.08
StudySTOP 0.62 (0.39, 0.99) 0.05
GA_samp 1.04 (0.96, 1.13) 0.34
HOMA-IR
Var Effect P
(Intercept) 6.49 (1.56, 26.94) 0.01
Folate_gp2≥40 0.76 (0.57, 1.00) 0.05
StudySTOP 0.49 (0.30, 0.81) 0.005
GA_samp 1.03 (0.94, 1.13) 0.51
Glucose (mmol/L)
Var Effect P
(Intercept) 6.85 (5.37, 8.74) <0.0001
Folate_gp2≥40 0.96 (0.88, 1.04) 0.30
StudySTOP 0.79 (0.73, 0.86) <0.0001
GA_samp 0.99 (0.97, 1.01) 0.53

38.5.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 70.73103 14.73149 52.79746 46.57671 107.4116
STOP 80.59937 13.34623 22.89233 57.21742 113.5364
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 61.21751 4.864402 119.13250 52.30512 71.64849
STOP 73.28061 3.084380 30.03457 67.24489 79.85809
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 1.900528 0.2060202 135.66754 1.533812 2.354921
STOP 1.754287 0.0924152 39.19691 1.577001 1.951503
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 29.75754 5.993665 Inf 20.05168 44.16144
STOP 18.50744 1.470135 Inf 15.83913 21.62526
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 8.117903 1.7585737 Inf 5.309442 12.411916
STOP 3.980335 0.3401272 Inf 3.366532 4.706048
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 6.143411 0.2573602 Inf 5.659147 6.669114
STOP 4.856323 0.1075299 Inf 4.650075 5.071717

38.5.4 Comparisons (Overall)

Hormone Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥40 / <40 1.13 (0.94, 1.34) 0.2
hPL (μg/ml) ≥40 / <40 1.04 (0.94, 1.16) 0.5
GH2 (ng/ml) ≥40 / <40 0.92 (0.80, 1.06) 0.3
Insulin (uU/ml) ≥40 / <40 0.79 (0.62, 1.02) 0.08
HOMA-IR ≥40 / <40 0.76 (0.57, 1.00) 0.05
Glucose (mmol/L) ≥40 / <40 0.96 (0.88, 1.04) 0.3

38.5.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

38.5.6 Plots